Simultaneous analysis of carbohydrates, polyols and amines in urine samples using chemical ionization gas chromatography with tandem mass spectrometry.
Róbert KubinecPeter KotoraViktória FerenczyJaroslav BlaškoPeter PodolecAlexandra Hengerics SzabóDarina BehúlováVáclav BierhanzlRadomír ČabalaStanislav StuchlíkWojciech FilipiakNgô Mạnh ThắngPublished in: Journal of separation science (2017)
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL-1 for mannitol to 4.7 mg/L for glucose.
Keyphrases
- gas chromatography
- tandem mass spectrometry
- ultra high performance liquid chromatography
- high performance liquid chromatography
- liquid chromatography
- mass spectrometry
- simultaneous determination
- high resolution mass spectrometry
- gas chromatography mass spectrometry
- solid phase extraction
- high resolution
- metabolic syndrome
- ms ms
- type diabetes
- amino acid
- blood pressure
- preterm birth
- preterm infants
- label free
- glycemic control