Comparison of Mitochondrial Superoxide Detection Ex Vivo/In Vivo by mitoSOX HPLC Method with Classical Assays in Three Different Animal Models of Oxidative Stress.
Sanela KalinovicMatthias OelzeSwenja Kröller-SchönSebastian StevenKsenija Vujacic-MirskiMiroslava KvandováIsabella SchmalAhmad Al ZuabiThomas MunzelAndreas DaiberPublished in: Antioxidants (Basel, Switzerland) (2019)
mitoSOX/HPLC represents a valid method for detection of mitochondrial superoxide formation in tissues of different animal disease models and correlates well with functional parameters and other markers of oxidative stress.
Keyphrases
- oxidative stress
- ms ms
- simultaneous determination
- diabetic rats
- dna damage
- ischemia reperfusion injury
- loop mediated isothermal amplification
- induced apoptosis
- hydrogen peroxide
- high performance liquid chromatography
- label free
- real time pcr
- solid phase extraction
- mass spectrometry
- gene expression
- tandem mass spectrometry
- heat shock
- signaling pathway
- liquid chromatography
- heat shock protein