A Critical Review of Analytical Methods for Quantification of Amphotericin B in Biological Samples and Pharmaceutical Formulations.
Gabriel Davi MarenaMatheus Aparecido Dos Santos RamosTais Maria BauabMarlus ChorilliPublished in: Critical reviews in analytical chemistry (2020)
Amphotericin B (AmB) is an important antifungal agent available in the clinical practice with the action mechanism related to the inhibition of ergosterol molecule present in the fungal cell wall. Given this, in order to expand AmB knowledge, this review article gathers important information of the AmB physical, chemical, and biological properties. In addition, the main analytical methods for quantifying and determining the AmB were also reported in this review, such as high-performance liquid chromatography (HPLC), liquid chromatography, tandem mass spectrophotometry (LC-MS/MS), immunoenzymatic assay (ELISA), capillary zone electrophoresis (CE) stands out and among others. Based in this review article, the scientific community will have important information to choose the best method for analysis in their scientific or clinical research, providing greater security and reliability in the obtained results.
Keyphrases
- high performance liquid chromatography
- liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- simultaneous determination
- cell wall
- solid phase extraction
- clinical practice
- healthcare
- mental health
- high resolution mass spectrometry
- ms ms
- physical activity
- gas chromatography
- public health
- candida albicans
- health information
- social media
- high resolution
- quantum dots