Antiproliferative, apoptosis-inducing, and GSTP1 demethylation activities of Ellagitannins isolated from Cornus alba L.
Siyeon JinSeoyeon SeonuJun YinSeyeon SonJinhyeok ChoiJaeyoon OhKyungmi KimMin-Won LeePublished in: Natural product research (2023)
This study aimed to prove the prostate cancer chemopreventive activity of compounds isolated from CA. We evaluated these compounds using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and evaluated their NF-κB inhibitory activity and apoptosis-inducing activity using western blot analysis and flow cytometry, respectively. Their DNA methylation activity was also evaluated via a methylation-specific polymerase chain reaction in androgen-dependent (LNCaP) and androgen-independent (PC-3) prostate cancer cell lines. Camptothin B ( 1 ), cornusiin B ( 2 ), and cornusiin A ( 3 ), which were isolated in our previous work, relatively reduced the protein expression levels in PCa cells. Among them, cornusiin B ( 2 ) exhibited excellent NF-κB inhibitory activity. Also, concentration-dependently increased the unmethylated DNA content and decreased the methylated DNA content in both PC-3 and LNCaP cells. Therefore, cornusiin B ( 2 ), which was isolated from CA, has the potential to act as a chemopreventive agent for prostate cancer.
Keyphrases
- prostate cancer
- cell cycle arrest
- pi k akt
- induced apoptosis
- oxidative stress
- radical prostatectomy
- cell death
- endoplasmic reticulum stress
- dna methylation
- signaling pathway
- flow cytometry
- circulating tumor
- lps induced
- cell free
- genome wide
- single molecule
- nuclear factor
- gene expression
- inflammatory response
- toll like receptor
- climate change