Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria.

Natália Ketrin Almeida-de-OliveiraOtacílio C MoreiraAline Rosa de LavigneLeila Mendonça-LimaGuilherme Loureiro WerneckCláudio Tadeu Daniel-RibeiroMaria de Fátima Ferreira-da-Cruz

Published in: Memorias do Instituto Oswaldo Cruz (2019)

Real-time PCR may replace conventional PCR in reference laboratories for P. vivax detection due to its rapidity. The TaqMan™ system is the most indicated when quantification assays are required. Performing tests in triplicate when diagnosing Plasmodium-infected-asymptomatic individuals is recommended to minimise diagnostic errors.

Keyphrases

real time pcr
plasmodium falciparum
high throughput
high resolution
patient safety
single cell
drug induced