A New Stilbene Glucoside from Biotransformation-Guided Purification of Chinese Herb Ha-Soo-Oh.
Jiumn-Yih WuHsiou-Yu DingTzi-Yuan WangMin-Hui HsuTe-Sheng ChangPublished in: Plants (Basel, Switzerland) (2022)
Ha-Soo-Oh is a traditional Chinese medicine prepared from the roots of Polygonum multiflorum Thunb. The herb extract has been widely used in Asian countries as a tonic agent and nutritional supplement for centuries. To identify new bioactive compounds in Chinese herbs, the biotransformation-guided purification (BGP) process was applied to Ha-Soo-Oh with Bacillus megaterium tyrosinase ( Bm TYR) as a biocatalyst. The result showed that a major biotransformed compound could be purified using the BGP process with preparative high-performance liquid chromatography (HPLC), and it was confirmed as a new compound, 2,3,5,3',4'-pentahydroxystilbene-2- O - β -glucoside (PSG) following mass and nucleic magnetic resonance (NMR) spectral analyses. PSG was further confirmed as a biotransformation product from 2,3,5,4'-tetrahydroxystilbene-2- O - β -glucoside (TSG) by Bm TYR. The new PSG exhibited 4.7-fold higher 1,1-diphenyl-2-picrylhydrazine (DPPH) free radical scavenging activity than that of TSG. The present study highlights the potential usage of BGP in herbs to discover new bioactive compounds in the future.
Keyphrases
- high performance liquid chromatography
- magnetic resonance
- simultaneous determination
- tandem mass spectrometry
- solid phase extraction
- mass spectrometry
- ms ms
- high resolution
- optical coherence tomography
- magnetic resonance imaging
- contrast enhanced
- current status
- risk assessment
- human health
- recombinant human
- bacillus subtilis
- hyaluronic acid