Effects of isoflurane and urethane anesthetics on glutamate neurotransmission in rat brain using in vivo amperometry.

KCl-evoked glutamate overflow was similar under urethane and isoflurane anesthesiaGlutamate clearance parameters were similar under both anesthesias in the cortex and hippocampus but not the thalamusGlutamate clearance kinetics differ between brain regions when anesthetized with urethane.Experimental design, brain region of interest, and outcome parameters of glutamate clearance should be considered when designing anesthetized amperometry recordings of glutamate.