FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants.

Junyan WangTao BingNan ZhangXiangjun Liu Dihua Shangguan

Published in: Chemical communications (Cambridge, England) (2021)

Herein, we report a FnCas12a/crRNA assisted Dumbbell-PCR method for the detection of isomiRs with double specificity and magnification. The single nucleotide variant of isomiRs in terminals and/or inner sequence could be discriminated by this strategy. Using this method, let-7a isomiRs in MCF-7 and MCF-7R cell lines were analyzed.

Keyphrases

real time pcr
breast cancer cells
loop mediated isothermal amplification
label free
copy number
gene expression
dna methylation
genome wide