Ultrasound-assisted dispersive micro-solid-phase extraction based on N-doped mesoporous carbon and high-performance liquid chromatographic determination of 1-hydroxypyrene in urine samples.
Fariborz OmidiMonireh KhademFatemeh DehghaniMirghani SeyedsomeahSeyed Jamaleddin ShahtaheriPublished in: Journal of separation science (2020)
In this research, a new ultrasound-assisted dispersive micro-solid-phase extraction method based on N-doped mesoporous carbon sorbent followed by high-performance liquid chromatography equipped with diode array detector for trace measurement of 1-hydroxypyrene as a metabolite of exposure to polycyclic aromatic hydrocarbons was optimized. Herein, the hard template method was used for the preparation of N-doped mesoporous carbon sorbent. The prepared sorbent was characterized using the Brunauer-Emmett-Teller method, transmission electron microscopy, and elemental analysis. Parameters affecting the extraction of the target metabolite were investigated using the Box-Behnken design method. Considering optimum parameters, the plotted calibration curve for 1-hydroxypyrene was linearly correlated with the concentration span of 0.1-50 μg/L for urine media. The accuracy of the optimized procedure was examined through the relative recovery tests on the fortified urine specimens. The relative recoveries fell between 95 and 101%. The method detection limit of the proposed procedure was also calculated to be 0.03 μg/L.
Keyphrases
- solid phase extraction
- high performance liquid chromatography
- molecularly imprinted
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- tandem mass spectrometry
- gas chromatography mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- gas chromatography
- quantum dots
- polycyclic aromatic hydrocarbons
- metal organic framework
- highly efficient
- mass spectrometry
- magnetic resonance imaging
- magnetic resonance
- high resolution
- high resolution mass spectrometry
- computed tomography
- minimally invasive
- electron microscopy
- sensitive detection