Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule.
Hidefumi IwashitaSatoru ToriiNoriyoshi NagahoraMunetaka IshiyamaKosei ShiojiKazumi SasamotoShigeomi ShimizuKentaro OkumaPublished in: ACS chemical biology (2017)
There has been a growing interest in mitophagy, mitochondria-selective autophagy, which plays an essential role in maintaining intracellular homeostasis. We have developed a small-molecule fluorescent probe, Mtphagy Dye, for visualizing mitophagy, which was readily synthesized from a known perylene derivative, perylene-3,4-dicarboxylic anhydride. Mtphagy Dye has suitable fluorescent properties for detecting mitochondrial acidification during mitophagy in the long-wavelength region that does not damage mitochondria. Using Mtphagy Dye, we were able to visualize mitophagy with both cases of Parkin-dependent and -independent HeLa cells.
Keyphrases
- living cells
- small molecule
- fluorescent probe
- oxidative stress
- cell death
- cell cycle arrest
- induced apoptosis
- nlrp inflammasome
- endoplasmic reticulum stress
- protein protein
- reactive oxygen species
- highly efficient
- signaling pathway
- single molecule
- quantum dots
- high resolution
- endoplasmic reticulum
- pi k akt
- visible light
- oxide nanoparticles