Application of a fluorescent H 2 S probe based on excited-state intramolecular proton transfer for detecting latent mechanism of H 2 S-induced MCF-7 apoptosis.

Background: H 2 S is the third gas transmitter affecting the growth, reproduction and survival of cancer cells. However, the H 2 S anticancer and antitumor mechanism still needs to be further studied. Methods: Here, FHS-1 was synthesized utilizing excited-state intramolecular proton transfer to detect H 2 S in MCF-7 cells, and investigated the effects of varying concentrations NaHS on apoptosis. Results: The study found that FHS-1 detects H 2 S levels with high selectivity and pH stability and that H 2 S may regulate apoptosis in MCF-7 cells through the p53/mTOR/STAT3 pathway. Conclusion: Researching the influence of H 2 S on apoptosis can serve as a theoretical foundation for future research into H 2 S-related anticancer medicines, and the H 2 S probe can be used as an effective cancer screening tool.