Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2'-deoxyguanosine in DNA.
Yoshiya KikukawaRyoji KawazoeRyo MiyaharaTakato SakuradaYusuke NagataShigeki SasakiYosuke TaniguchiPublished in: Chemical communications (Cambridge, England) (2022)
The identification of the position of 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA is important to clarify the pathogenesis of many diseases. We herein developed a purine-1,3-diazaphenoxazine triphosphate (dPdapTP) and described the first example of detecting the presence of 8-oxo-dG by amplifying it several hundred times after the multiple-turnover single nucleotide primer extension reactions.