Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4.
Thuy P DaoAnitha RajendranSarasi K K GalagederaWilliam HawsCarlos A CastañedaPublished in: bioRxiv : the preprint server for biology (2023)
1) Full-length UBQLN1, UBQLN2 and UBQLN4 all exhibit LCST phase transitions but to different degrees.2) The N-terminal region (N-terminal to the UBL) substantially regulates UBQLN phase separation.3) Removal of the disease-associated proline-rich (Pxx) region in UBQLN2 removes the strong temperature dependence of UBQLN2 phase separation.
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