Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela.
Ivonne MoralesKerstin Daniela RosenbergerTereza MagalhaesClarice Neuenschwander Lins de MoraisMaria Cynthia BragaErnesto Torres de Azevedo MarquesGuilherme Amaral CalvetLuana Santana DamascenoPatricia BrasilAna Maria Bispo de FilippsAdriana TamiSarah BethencourtMayling AlvarezPedro A MartínezMaria G GuzmanBruno Souza BenevidesAndrea CapraraNguyen Than Ha QuyenCameron P SimmonsBridget A WillsXavier de LamballerieJan Felix DrexlerThomas Jaenischnull nullPublished in: PLoS neglected tropical diseases (2021)
Our findings exemplify the challenges of the assessment of test performance for ZIKV serological tests in the real-world setting, during co-circulation of DENV, ZIKV, and CHIKV. However, we can also demonstrate that the IgAM immunoassay exhibits superior sensitivity to detect ZIKV RT-PCR confirmed infections compared to IgG and IgM immunoassays. The IgAM assay also proves to be promising for detection of anti-ZIKV seroconversions in sequential samples, both in ZIKV PCR-positive as well as PCR-negative patients, making this a candidate assay for serological monitoring of pregnant women in future ZIKV outbreaks.