Dual-target, real-time PCR for the diagnosis of intraocular Toxoplasma gondii infections.
Carlos A GomezMalaya K SahooGhazala Yasmeen KahnLina ZhongJosé G MontoyaBenjamin A PinskyThuy DoanPublished in: The British journal of ophthalmology (2019)
This single-reaction, internally controlled, dual-target (B1, Rep529) real-time PCR for the detection of T. gondii DNA in intraocular specimens demonstrated excellent agreement with nested, conventional, B1 PCR. The dual-target design may ensure T. gondii detection when variation is present in one of two target regions.