Biosynthesis of glyceride glycoside (nonionic surfactant) by amylosucrase, a powerful glycosyltransferase.
Ye-Jin KimInonge Noni SiziyaSeungpyo HongGil-Yong LeeMyung-Ji SeoYoung-Rok KimSang-Ho YooCheon-Seok ParkDong-Ho SeoPublished in: Food science and biotechnology (2021)
Amylosucrase (ASase, E.C. 2.4.1.4) is a powerful transglycosylation enzyme that can transfer glucose from sucrose to the hydroxyl (-OH) group of various compounds. In this study, recombinant ASases from Deinococcus geothermalis (DgAS) and Bifidobacterium thermophilum (BtAS) were used to synthesize biosurfactants based on the computational analysis of predicted docking simulations. Successful predictions of the binding affinities, conformations, and three-dimensional structures of three surfactants were computed from receptor-ligand binding modes. DgAS and BtAS were effective in the synthesis of biosurfactants from glyceryl caprylate, glyceryl caprate, and polyglyceryl-2 caprate. The results of the transglycosylation reaction were consistent for both ASases, with glyceryl caprylate acceptor showing the highest concentration, as confirmed by thin layer chromatography. Furthermore, the transglycosylation reactions of DgAS were more effective than those of BtAS. Among the three substrates, glyceryl caprylate glycoside and glyceryl caprate glycoside were successfully purified by liquid chromatography-mass spectrometry (LC-MS) with the corresponding molecular weights.
Keyphrases
- mass spectrometry
- liquid chromatography
- tandem mass spectrometry
- high resolution mass spectrometry
- high resolution
- molecular dynamics
- high performance liquid chromatography
- gas chromatography
- capillary electrophoresis
- magnetic resonance imaging
- high speed
- simultaneous determination
- binding protein
- solid phase extraction
- metabolic syndrome
- adipose tissue
- transcription factor
- blood glucose
- cell free
- insulin resistance
- dna binding
- diffusion weighted imaging