Biomarker Discovery in Patients with Immunotherapy-Treated Melanoma with Imaging Mass Cytometry.

Sandra Martinez-MorillaFranz Villarroel-EspindolaPok Fai WongMaria I TokiThazin Nwe Aung Vasiliki PelekanouBrian Bourke-MartinKurt A SchalperHarriet M KlugerDavid L Rimm

Published in: Clinical cancer research : an official journal of the American Association for Cancer Research (2021)

Using digital image analysis based on pixel colocalization to assess IMC data allowed us to quantitively measure 25 markers simultaneously on formalin-fixed, paraffin-embedded tissue microarray samples. In addition to showing high concordance with other multiplexing technologies, we identified a series of potentially indicative biomarkers for immunotherapy in metastatic melanoma, including B2M.

Keyphrases

high resolution
small molecule
single cell
electronic health record
high throughput
big data
skin cancer
newly diagnosed
deep learning