Comparison of an in-house 'home-brew' and commercial ViroSeq integrase genotyping assays on HIV-1 subtype C samples.

Kaelo K SeatlaWonderful T ChogaMompati MogweleThabo DiphokoDorcas MaruapulaLucy MupfumiRosemary M MusondaChristopher F RowleyAva AvalosIshmael KasvosveSikhulile MoyoSimani Gaseitsiwe

Published in: PloS one (2019)

The IH-Int assay had a high amplification success rate and high concordance with the commercial assay. It is significantly cheaper compared to the commercial assay. Our assay has the needed specifications for routine monitoring of participants on Dolutegravir based regimens in Botswana.

Keyphrases

high throughput
antiretroviral therapy
healthcare
human immunodeficiency virus
hiv infected
hiv positive
genome wide
dna methylation
hiv infected patients