Novel Circulating Hypermethylated RASSF1A ddPCR for Liquid Biopsies in Patients With Pediatric Solid Tumors.
Lieke M J van ZogchelNathalie S M LakOnno J H M VerhagenAhmed TissoudaliMohammed Gussmalla NuruNina U GelineauLily Zappeij-KannengieterAhmad JavadiEline A M ZijtregtopJohannes Hendrikus Maria MerksMarry van den Heuvel-EibrinkAntoinette Y N Schouten-van MeeterenJanine StutterheimC Ellen van der SchootGodelieve A M TytgatPublished in: JCO precision oncology (2021)
Our findings demonstrate the value of ddPCR-based detection of hypermethylated RASSF1A as a circulating molecular tumor marker in neuroblastoma. Our preliminary investigation of RASSF1A hypermethylation detection in circulating cfDNA of other pediatric tumor entities demonstrates potential as a pan-tumor marker, but requires investigation in larger cohorts to evaluate its use and limitations.