Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13.
Olga PaciosLaura Fernandez-GarciaInés BleriotLucia BlascoAntón AmbroaMaría LópezConcha Ortiz-CartagenaFelipe Fernández CuencaJesús Oteo-IglesiasAlvaro PascualLuis Martinez-MartinezPilar Domingo-CalapMaria TomasPublished in: Viruses (2021)
Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.
Keyphrases
- multidrug resistant
- klebsiella pneumoniae
- escherichia coli
- gram negative
- amino acid
- drug resistant
- acinetobacter baumannii
- end stage renal disease
- single cell
- stem cells
- ejection fraction
- chronic kidney disease
- endothelial cells
- gene expression
- pseudomonas aeruginosa
- intensive care unit
- cell therapy
- mesenchymal stem cells
- climate change
- protein protein
- mass spectrometry