Ceftazidime-Decorated Gold Nanoparticles: a Promising Strategy against Clinical Ceftazidime-Avibactam-Resistant Enterobacteriaceae with Different Resistance Mechanisms.
Zeyu HuangHaifeng LiuXiaotuan ZhangMiran TangYuzhan LinLuozhu FengJianzhong YeZhexiao MaLijiang ChenPublished in: Antimicrobial agents and chemotherapy (2023)
Nanoparticle-based antibiotic delivery systems are essential in combating antibiotic-resistant bacterial infections arising from acquired resistance and/or biofilm formation. Here, we report that the ceftazidime-decorated gold nanoparticles (CAZ_Au NPs) can effectively kill clinical ceftazidime-avibactam-resistant Enterobacteriaceae with various resistance mechanisms. Further study of underlying antibacterial mechanisms suggests that CAZ_Au NPs can damage the bacterial cell membrane and increase the level of intracellular reactive oxygen species. Moreover, CAZ_Au NPs show great potential in inhibiting biofilm formation and eradicating mature biofilms via crystal violet and scanning electron microscope assays. In addition, CAZ_Au NPs demonstrate excellent performance in improving the survival rate in the mouse model of abdominal infection. In addition, CAZ_Au NPs show no significant toxicity at bactericidal concentrations in the cell viability assay. Thus, this strategy provides a simple way to drastically improve the potency of ceftazidime as an antibiotic and its use in further biomedical applications.
Keyphrases
- reduced graphene oxide
- biofilm formation
- gold nanoparticles
- gram negative
- pseudomonas aeruginosa
- multidrug resistant
- candida albicans
- staphylococcus aureus
- sensitive detection
- oxide nanoparticles
- reactive oxygen species
- klebsiella pneumoniae
- escherichia coli
- mouse model
- cystic fibrosis
- oxidative stress
- high throughput
- quantum dots
- visible light
- high resolution
- electron microscopy
- free survival
- urinary tract infection
- human health
- solid state
- wound healing