In vivo creation of plasmid pCRT01 and its use for the construction of carotenoid-producing Paracoccus spp. strains that grow efficiently on industrial wastes.

Anna MajLukasz DziewitLukasz DrewniakMaciej GarstkaTomasz KruconKatarzyna PiatkowskaKatarzyna GieczewskaJakub CzarneckiEwa FurmanczykRobert LasekJadwiga BajDariusz Bartosik

Published in: Microbial cell factories (2020)

We demonstrated a new approach for the construction of carotenoid-producing bacterial strains which relies on a single plasmid-mediated transfer of a pigment synthesis gene locus between Paracoccus strains. This strategy facilitates screening for producer strains in terms of synthesis efficiency, pigment profile and ability to grow on low-cost industrial waste-based media, which should increase the cost-effectiveness of microbial production of carotenoids.

Keyphrases

escherichia coli
low cost
heavy metals
wastewater treatment
risk assessment
gene expression
copy number
dna methylation
high density
genetic diversity