Breadmaking-inspired Antioxidant Porous Yeast Microcarriers for Stem Cell Delivery in Diabetic Wound Treatment.

Stem cell-based therapies have exhibited significant promise in the treatment of diabetic ulcers (DU). Nevertheless, enhancing the survival rate and functionality of transplanted stem cells poses a substantial challenge. In this study, inspired by the breadmaking process, we have devised yeast microcarriers (YMC) as vehicles for stem cells to address these challenges. The fabrication of YMC involved the amalgamation of microfluidic emulsification with yeast-mediated fermentation, yielding microcarriers with outstanding biocompatibility, high porosity, and antioxidant activity. Adipose-derived stem cells (ADSCs) seeded onto YMC displayed remarkable cell viability and retained their cellular functions effectively. Additionally, YMC boasted a rich glutathione (GSH) content and exhibited remarkable ROS scavenging ability, thus shielding the ADSCs from oxidative stress. In vivo experiments further substantiated that ADSC@YMC implementation significantly lowered ROS levels in diabetic wounds, resulting in enhanced stem cell retention and improved angiogenesis, collagen deposition, and tissue regeneration. These results highlighted the potential of ADSC@YMC as a promising platform for delivering stem cell in the treatment of diabetic wounds. This article is protected by copyright. All rights reserved.