Sequential and counter-selectable cassettes for fission yeast.

Hanna AmelinaVera MoiseevaLaura Catharine CollopySiân Rosanna PearsonChristine Anne ArmstrongKazunori Tomita

Published in: BMC biotechnology (2016)

The plasmid constructs and techniques described here are invaluable tools for sequential gene targeting and will simplify construction of fission yeast strains required for study.

Keyphrases

escherichia coli
saccharomyces cerevisiae
crispr cas
copy number
genome wide
cancer therapy
gene expression
transcription factor
genome wide identification