Sargassum horneri (Turner) C. Agardh Extract Regulates Neuroinflammation In Vitro and In Vivo.
Jun Hwi ChoDae Hyun KimJong Suk LeeMi-Suk SeoMi Eun KimJun Sik LeePublished in: Current issues in molecular biology (2022)
Previously, we reported that Sargassum horneri (Turner) C. Agardh ( S. horneri ) is a brown algae species that exerts anti-inflammatory activity toward murine macrophages. However, the anti-neuroinflammatory effects and the mechanism of S. horneri on microglia cells are still unknown. We investigated the anti-neuroinflammatory effects of S. horneri extract on microglia in vitro and in vivo. In the present study, we found that S. horneri was not cytotoxic to BV-2 microglia cells and it significantly decreased lipopolysaccharide (LPS)-induced NO production. Moreover, S. horneri also diminished the protein expression of iNOS, COX-2, and cytokine production, including IL-1β, TNF-α, and IL-6, on LPS-stimulated microglia activation. S. horneri elicited anti-neuroinflammatory effects by inhibiting phosphorylation of p38 MAPK and NF-κB. In addition, S. horneri inhibited astrocytes and microglia activation in LPS-challenged mice brain. Therefore, these results suggested that S. horneri exerted anti-neuroinflammatory effects on LPS-stimulated microglia cell activation by inhibiting neuroinflammatory factors and NF-κB signaling.
Keyphrases
- inflammatory response
- lps induced
- lipopolysaccharide induced
- toll like receptor
- signaling pathway
- induced apoptosis
- oxidative stress
- neuropathic pain
- anti inflammatory
- cell cycle arrest
- single cell
- rheumatoid arthritis
- nitric oxide
- type diabetes
- spinal cord
- cell death
- traumatic brain injury
- nuclear factor
- brain injury
- bone marrow
- mass spectrometry
- pi k akt
- functional connectivity
- skeletal muscle
- white matter
- resting state
- subarachnoid hemorrhage
- cognitive impairment