A novel near-infrared fluorescent probe based on isophorone for the bioassay of endogenous cysteine.
Hong-Bo LiuHai XuXin GuoJian XiaoZheng-Hong CaiYa-Wen WangYu PengPublished in: Organic & biomolecular chemistry (2021)
A dicyanoisophorone/acrylate-combined probe (DDP) was synthesized and designed as a near-infrared (NIR) fluorescent sensor for the rapid identification of Cys over Hcy and GSH in aqueous solution with a large Stokes shift (143 nm). The detection limit of Cys was 1.23 μM, which was lower than that of the intracellular Cys concentration. DDP was cell membrane-permeable and had been successfully applied to the detection of intracellular Cys in HeLa cells. The detection mechanism was determined by 1H NMR titration, MS and DFT calculations.
Keyphrases
- fluorescent probe
- living cells
- loop mediated isothermal amplification
- label free
- aqueous solution
- real time pcr
- cell cycle arrest
- density functional theory
- induced apoptosis
- magnetic resonance
- photodynamic therapy
- multiple sclerosis
- ms ms
- mass spectrometry
- quantum dots
- reactive oxygen species
- sensitive detection
- molecular docking
- oxidative stress
- molecular dynamics
- cell death
- solid state
- crystal structure