Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves.

Drought intensity modifies the assimilatory pathway of glutathione (GSH) synthesis. Abscisic acid (ABA) is a representative signaling hormone involved in regulating plant stress responses. This study aimed to investigate an interactive regulation of sulfate and/or ABA in GSH metabolism and redox. The drought-responsive alterations in sulfate assimilation and GSH-based redox reactions were assessed relative to ABA responses on the time-course of drought intensity. Drought-responsive H 2 O 2 concentrations were divided into two distinct phases-an initial 4 days of no change ( Ψ w ≥ -0.49 MPa) and a phase of higher accumulation during the late phase of the drought (days 10-14; Ψ w ≤ -1.34 MPa). During the early phase of the drought, GSH/GSSG redox state turned to the slightly reduced state with a transient increase in GSH, resulting from a strong activation of H 2 O 2 scavenging enzymes, ascorbate peroxidase (APOX) and glutathione reductase (GR). The late phase of the drought was characterized by a decrease in GSH due to cysteine accumulation, shifting GSH- and NADPH-based redox states to higher oxidization, increasing sulfate and ABA in xylem, and causing ABA accumulation in leaves. Regression analysis revealed that sulfate in xylem sap was positively correlated with H 2 O 2 concentrations and ABA was closely related to decreases in the GSH pool and the oxidation of GSH catalyzed by glutathione peroxidase (GPOX). These results indicate that drought-induced oxidation proceeds through the suppression of GSH synthesis and further GSH oxidation in a sulfate-activated ABA-dependent manner.