Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique.
Amizon AzizanJochen BüchsPublished in: Journal of biological engineering (2017)
The 3D liquid distribution data collected at varying filling volume and shaking frequency, comprising of LB and TB values relative to the direction of the centrifugal acceleration are essential for validating future numerical solutions using CFD to predict vital engineering parameters in shake flask.