New Insights into the Structure and Assembly of Bacteriophage P1.
Miguel F GonzalesDenish K PiyaBrian KoehlerKailun ZhangZihao YuLanying ZengJason J GillPublished in: Viruses (2022)
Bacteriophage P1 is the premier transducing phage of E. coli . Despite its prominence in advancing E. coli genetics, modern molecular techniques have not been applied to thoroughly understand P1 structure. Here, we report the proteome of the P1 virion as determined by liquid chromatography tandem mass-spectrometry. Additionally, a library of single-gene knockouts identified the following five previously unknown essential genes: pmgA , pmgB , pmgC , pmgG , and pmgR . In addition, proteolytic processing of the major capsid protein is a known feature of P1 morphogenesis, and we identified the processing site by N-terminal sequencing to be between E120 and S121, producing a 448-residue, 49.3 kDa mature peptide. Furthermore, the P1 defense against restriction (Dar) system consists of six known proteins that are incorporated into the virion during morphogenesis. The largest of these, DarB, is a 250 kDa protein that is believed to translocate into the cell during infection. DarB deletions indicated the presence of an N-terminal packaging signal, and the N-terminal 30 residues of DarB are shown to be sufficient for directing a heterologous reporter protein to the capsid. Taken together, the data expand on essential structural P1 proteins as well as introduces P1 as a nanomachine for cellular delivery.
Keyphrases
- liquid chromatography tandem mass spectrometry
- escherichia coli
- amino acid
- single cell
- protein protein
- genome wide
- simultaneous determination
- binding protein
- heat shock protein
- ms ms
- solid phase extraction
- gene expression
- crispr cas
- cell therapy
- electronic health record
- deep learning
- copy number
- mass spectrometry
- mesenchymal stem cells
- dna methylation
- cystic fibrosis
- neural network
- bioinformatics analysis
- saccharomyces cerevisiae