A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue.
Emanuele RizzutoSilvia CarosioMartina FaraldiSimona PisuAntonio MusaròZaccaria Del PretePublished in: Applied bionics and biomechanics (2016)
Tissue engineering is a multidisciplinary science based on the application of engineering approaches to biologic tissue formation. Engineered tissue internal organization represents a key aspect to increase biofunctionality before transplant and, as regarding skeletal muscles, the potential of generating contractile forces is dependent on the internal fiber organization and is reflected by some macroscopic parameters, such as the spontaneous contraction. Here we propose the application of digital image correlation (DIC) as an independent tool for an accurate and noninvasive measurement of engineered muscle tissue spontaneous contraction. To validate the proposed technique we referred to the X-MET, a promising 3-dimensional model of skeletal muscle. The images acquired through a high speed camera were correlated with a custom-made algorithm and the longitudinal strain predictions were employed for measuring the spontaneous contraction. The spontaneous contraction reference values were obtained by studying the force response. The relative error between the spontaneous contraction frequencies computed in both ways was always lower than 0.15%. In conclusion, the use of a DIC based system allows for an accurate and noninvasive measurement of biological tissues' spontaneous contraction, in addition to the measurement of tissue strain field on any desired region of interest during electrical stimulation.
Keyphrases
- skeletal muscle
- smooth muscle
- high speed
- deep learning
- insulin resistance
- public health
- gene expression
- spinal cord injury
- high resolution
- rheumatoid arthritis
- atomic force microscopy
- type diabetes
- magnetic resonance imaging
- convolutional neural network
- computed tomography
- risk assessment
- metabolic syndrome
- magnetic resonance
- adipose tissue
- climate change
- optical coherence tomography
- quality improvement
- diffusion weighted imaging