Long-read single-cell sequencing reveals the transcriptional landscape of spermatogenesis in obstructive azoospermia and sertoli cell-only patients.

We have identified total full-length transcripts in OA and NOA, and new genes were found. Furthermore, specific expressed full-length transcripts were detected, and the genomic structure of transcripts was mapped in different cell types. These findings may provide valuable information on human spermatogenesis and the treatment of male infertility.