Ala-geninthiocin, a new broad spectrum thiopeptide antibiotic, produced by a marine Streptomyces sp. ICN19.
Appadurai Muthamil IniyanEnge SudarmanJoachim WinkRajaretinam Rajesh KannanSamuel Gnana Prakash VincentPublished in: The Journal of antibiotics (2018)
Bioassay-guided screening of antibacterial compounds from the cultured marine Streptomyces sp. ICN19 provided Ala-geninthiocin (1), along with its known analogs geninthiocin (2) and Val-geninthiocin (3) and the indolocarbazole staurosporine (4). The structure of 1 was determined on the basis of 1D and 2D NMR spectra and ESI-HRMS. The absolute configurations of the amino acid residues were determined by enantioselective GC-MS analysis. Compound 1 exhibited potent activity against Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, Mycobacterium smegmatis, and Micrococcus luteus, as well as cytotoxicity against A549 human lung carcinoma cells with an IC50 value of 6 nM.
Keyphrases
- bacillus subtilis
- staphylococcus aureus
- amino acid
- ms ms
- magnetic resonance
- mycobacterium tuberculosis
- endothelial cells
- high resolution
- anti inflammatory
- photodynamic therapy
- gram negative
- molecular docking
- solid state
- silver nanoparticles
- density functional theory
- cystic fibrosis
- mass spectrometry
- molecular dynamics simulations