2'-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA.

Sabrina M HuberPieter van DelftArun TanpureEric A MiskaSamantha Kendrick

Published in: Journal of the American Chemical Society (2017)

5-Hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.

Keyphrases

nucleic acid
small molecule
single cell
high throughput
mass spectrometry
gram negative
atomic force microscopy
high speed
tandem mass spectrometry