Diosmin Treats Lipopolysaccharide-Induced Inflammatory Pain and Peritonitis by Blocking NF-κB Activation in Mice.
Victor FattoriFernanda S Rasquel-OliveiraNayara A ArteroCamila R FerrazSergio M BorghiRúbia CasagrandeWaldiceu Aparecido VerriPublished in: Journal of natural products (2020)
Gram-negative bacterial infections induce inflammation and pain. Lipopolysaccharide (LPS) is a pathogen-associated molecular pattern and the major constituent of Gram-negative bacterial cell walls. Diosmin is a citrus flavonoid with antioxidant and anti-inflammatory activities. Here we investigated the efficacy of diosmin in a nonsterile model of inflammatory pain and peritonitis induced by LPS. Diosmin reduced in a dose-dependent manner LPS-induced inflammatory mechanical hyperalgesia, thermal hyperalgesia, and neutrophil recruitment to the paw (myeloperoxidase activity). Diosmin also normalized changes in paw weight distribution assessed by static weight bearing as a nonreflexive method of pain measurement. Moreover, treatment with diosmin inhibited LPS-induced peritonitis as observed by a reduction of leukocyte recruitment and oxidative stress. Diosmin reduced LPS-induced total ROS production (DCFDA assay) and superoxide anion production (NBT assay and NBT-positive cells). We also observed a reduction of LPS-induced oxidative stress and cytokine production (IL-1β, TNF-α, and IL-6) in the paw. Furthermore, we demonstrated that diosmin inhibited LPS-induced NF-κB activation in peritoneal exudate. Thus, we demonstrated, using a model of nonsterile inflammation induced by LPS, that diosmin is a promising molecule for the treatment of inflammation and pain.
Keyphrases
- lps induced
- inflammatory response
- oxidative stress
- lipopolysaccharide induced
- gram negative
- neuropathic pain
- chronic pain
- anti inflammatory
- toll like receptor
- pain management
- multidrug resistant
- induced apoptosis
- dna damage
- body mass index
- diabetic rats
- spinal cord
- rheumatoid arthritis
- high throughput
- signaling pathway
- weight loss
- cell therapy
- single cell
- metabolic syndrome
- postoperative pain
- bone marrow
- combination therapy
- ionic liquid
- high resolution
- heat shock
- wild type
- insulin resistance
- high speed