Digital Quantification of Chemical Oligonucleotide Synthesis Errors.
Stefan FilgesPia MouhannaAnders StåhlbergPublished in: Clinical chemistry (2022)
Our data demonstrate that manufacturer, synthesis strategy, purity, batch, and sequence context all contribute to errors in chemically synthesized oligonucleotides and need to be considered when choosing and evaluating oligonucleotides. High-performance oligonucleotides are essential in numerous molecular applications, including clinical diagnostics.