INFLECT: an R-package for cytometry cluster evaluation using marker modality.
Jan VerhoeffSanne AbelnJuan-Jesús García-VallejoPublished in: BMC bioinformatics (2022)
INFLECT addresses a knowledge gap in high-dimensional cytometry analysis, namely assessing clustering results. This is done through monitoring marker distributions for interquartile range and unimodality across a range of metacluster numbers. The inflection point is the optimal trade-off between cellular heterogeneity and statistical power, applied in this work for FlowSOM clustering on mass cytometry datasets.
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