Actin nano-architecture of phagocytic podosomes.
John Cody HerronShiqiong HuTakashi WatanabeAna T NogueiraBei LiuMegan E KernJesse Scott AaronAaron TaylorMichael PabloTeng-Leong ChewTimothy C ElstonKlaus M HahnPublished in: Nature communications (2022)
Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we characterize the structure and organization of phagocytic podosomes using interferometric photoactivated localization microscopy, a super-resolution microscopy technique capable of 15-20 nm resolution, together with structured illumination microscopy and localization-based super-resolution microscopy. Phagocytic podosomes are observed during frustrated phagocytosis, a model in which cells attempt to engulf micropatterned IgG antibodies. For circular patterns, this results in regular arrays of podosomes with well-defined geometry. Using persistent homology, we develop a pipeline for semi-automatic identification and measurement of podosome features. These studies reveal an hourglass shape of the podosome actin core, a protruding knob at the bottom of the core, and two actin networks extending from the core. Additionally, the distributions of paxillin, talin, myosin II, α-actinin, cortactin, and microtubules relative to actin are characterized.
Keyphrases
- single molecule
- high resolution
- cell migration
- high speed
- high throughput
- optical coherence tomography
- label free
- induced apoptosis
- machine learning
- photodynamic therapy
- gene expression
- oxidative stress
- cell proliferation
- genome wide
- mass spectrometry
- body composition
- bone mineral density
- pseudomonas aeruginosa
- high density
- endoplasmic reticulum stress
- binding protein
- bone regeneration
- bone loss
- pi k akt