DNA Strand Breaks and Denaturation as Probes of Chemical Reactivity versus Thermal Effects of Atmospheric Pressure Plasma Jets.
Amal SebastianDaniel LipaSylwia PtasinskaPublished in: ACS omega (2022)
An atmospheric pressure plasma jet (APPJ) is being advanced as an alternative radiation type that offers excellent efficacy in an array of medical applications against specific biological targets such as DNA. This work explores the possibility of implementing DNA and its damage as a probe for specific plasma diagnostics such as reactive plasma species formation and transient local heating. We analyzed both APPJ characteristics based on the detection of plasma-induced strand breaks and DNA denaturation. Further, we implemented a machine learning model based on artificial neural networks to predict the type and extent of DNA damage for a given combination of APPJ parameter values. This methodology is an important step toward deciphering and explaining the potential adverse effects of APPJ on biological samples of any prospective interest in medicine.
Keyphrases
- circulating tumor
- single molecule
- machine learning
- dna damage
- cell free
- healthcare
- oxidative stress
- small molecule
- blood brain barrier
- artificial intelligence
- living cells
- high frequency
- quantum dots
- risk assessment
- deep learning
- single cell
- high throughput
- fluorescence imaging
- endothelial cells
- drug induced
- fluorescent probe
- sensitive detection