CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation.
Huimin ChenKe HuYing XieYucheng QiWenjuan LiYao-Hui HeShijie FanWen LiuChenghua LiPublished in: International journal of molecular sciences (2022)
∆Np63α is a key transcription factor overexpressed in types of squamous cell carcinomas (SCCs), which represses epithelial-mesenchymal transition (EMT) and cell migration. In this study, we found that CDK1 phosphorylates ∆Np63α at the T123 site, impairing its affinity to the target promoters of its downstream genes and its regulation of them in turn. Database analysis revealed that CDK1 is overexpressed in head and neck squamous cell carcinomas (HNSCCs), especially the metastatic HNSCCs, and is negatively correlated with overall survival. We further found that CDK1 promotes the EMT and migration of HNSCC cells by inhibiting ∆Np63α. Altogether, our study identified CDK1 as a novel regulator of ΔNp63α, which can modulate EMT and cell migration in HNSCCs. Our findings will help to elucidate the migration mechanism of HNSCC cells.
Keyphrases
- epithelial mesenchymal transition
- cell migration
- squamous cell
- cell cycle
- signaling pathway
- induced apoptosis
- transcription factor
- transforming growth factor
- high grade
- cell proliferation
- emergency department
- dna methylation
- gene expression
- single cell
- mass spectrometry
- electronic health record
- fluorescent probe
- adverse drug
- living cells
- genome wide analysis