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In Situ Construction of Protein-Based Semisynthetic Biosensors.

Tsuyoshi UedaTomonori TamuraItaru Hamachi
Published in: ACS sensors (2018)
Chemically constructed biosensors consisting of a protein scaffold and an artificial small molecule have recently been recognized as attractive analytical tools for the specific detection and real-time monitoring of various biological substances or events in cells. Conventionally, such semisynthetic biosensors have been prepared in test tubes and then introduced into cells using invasive methods. With the impressive advances seen in bioorthogonal protein conjugation methodologies, however, it is now becoming feasible to directly construct semisynthetic biosensors in living cells, providing unprecedented tools for life-science research. We discuss here recent efforts regarding the in situ construction of protein-based semisynthetic biosensors and highlight their uses in the visualization and quantification of biomolecules and events in multimolecular and crowded cellular systems.
Keyphrases
  • small molecule
  • protein protein
  • induced apoptosis
  • living cells
  • label free
  • amino acid
  • cell cycle arrest
  • public health
  • wastewater treatment
  • liquid chromatography
  • tissue engineering