EMab-300 Detects Mouse Epidermal Growth Factor Receptor-Expressing Cancer Cell Lines in Flow Cytometry.
Nohara GotoHiroyuki SuzukiTomohiro TanakaKenichiro IshikawaTsunenori OuchidaMika K KanekoYukinari KatoPublished in: Antibodies (Basel, Switzerland) (2023)
Epidermal Growth Factor Receptor (EGFR) overexpression or its mutation mediates the sustaining proliferative signaling, which is an important hallmark of cancer. Human EGFR-targeting monoclonal antibody (mAb) therapy such as cetuximab has been approved for clinical use in patients with colorectal cancers and head and neck squamous cell carcinomas. A reliable preclinical mouse model is essential to further develop the mAb therapy against EGFR. Therefore, sensitive mAbs against mouse EGFR (mEGFR) should be established. In this study, we developed a specific and sensitive mAb for mEGFR using the Cell-Based Immunization and Screening (CBIS) method. The established anti-mEGFR mAb, EMab-300 (rat IgG 1 , kappa), reacted with mEGFR-overexpressed Chinese hamster ovary-K1 (CHO/mEGFR) and endogenously mEGFR-expressed cell lines, including NMuMG (a mouse mammary gland epithelial cell) and Lewis lung carcinoma cells, using flow cytometry. The kinetic analysis using flow cytometry indicated that the K D of EMab-300 for CHO/mEGFR and NMuMG was 4.3 × 10 -8 M and 1.9 × 10 -8 M, respectively. These results indicated that EMab-300 applies to the detection of mEGFR using flow cytometry and may be useful to obtain the proof of concept in preclinical studies.
Keyphrases
- flow cytometry
- epidermal growth factor receptor
- monoclonal antibody
- squamous cell
- tyrosine kinase
- advanced non small cell lung cancer
- cell therapy
- papillary thyroid
- mouse model
- small cell lung cancer
- single cell
- cell proliferation
- stem cells
- squamous cell carcinoma
- inflammatory response
- transcription factor
- childhood cancer
- high grade
- cancer therapy
- mesenchymal stem cells
- toll like receptor
- quantum dots
- lymph node metastasis
- real time pcr
- rectal cancer
- smoking cessation