In this article, a multivariate analysis of the parameters determining the transport and fate of selected heavy metals in the water - bottom sediment interface was carried out. The studies were carried out in the summer season of 2019 at Nielisz Reservoir (southeastern Poland, Lublin Voivodeship). Finally, a previously unknown factor related to the quality of organic matter was identified. Autochthonous organic matter was shown to promote the accumulation of the studied heavy metals. To date, the significance of the origin of organic matter in the context of the transport and fate of heavy metals in retention reservoirs has rarely been reported in the scientific literature. More than that, this factor was not considered an important component in the process of heavy metal deposition in bottom sediments. However, it turns out that not only the quantity of organic matter, but also its quality plays an important role in the circulation of heavy metals in retention reservoir ecosystems. It was found that autochthonous organic matter promotes the accumulation of the studied heavy metals. It can be assumed that, in a sense, it plays the role of a catenary ("hub") controlling the fate of heavy metals in the water-sediment system. It has also been conjectured that, in a sense, OM S may reflect the potential for heavy metal assimilation by aquatic vascular plants (mainly of the C3 group). Plants with a photosynthetic pathway similar to the C3 group generally have a much lower enrichment in the 13 C isotope (δ 13 C from -38‰ to -22‰). In our case, the lowest δ 13 C-TOC S value was -24.05‰, and the average for the whole reservoir was -21.53‰. In addition, it was observed that quantitative changes in the isotopic composition of total organic carbon δ 13 C-TOC S , corresponded with changes in the content of the heavy metals studied in entrapped sediments.