Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells.
Jonathan TysonKevin HuShuai ZhengPhylicia KiddNeville DadinaLing ChuDerek ToomreJoerg BewersdorfAlanna SchepartzPublished in: ACS central science (2021)
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
Keyphrases
- single molecule
- fluorescent probe
- living cells
- energy transfer
- quantum dots
- light emitting
- induced apoptosis
- flow cytometry
- atomic force microscopy
- cell cycle arrest
- endoplasmic reticulum
- cell death
- ionic liquid
- reactive oxygen species
- high speed
- sensitive detection
- endoplasmic reticulum stress
- signaling pathway
- high resolution
- oxidative stress
- pi k akt
- public health
- single cell