Urinary MicroRNA Sensing Using Electrochemical Biosensor to Evaluate Colorectal Cancer Progression.
Sow-Neng PangYu-Lun LinYueh-Er ChiouWai-Hung LeungWen-Hui WengPublished in: Biomedicines (2022)
Research in cancer diagnostics has recently established its footing and significance in the biosensor sphere, emphasizing the idea of a unique probe design used as a sensor and actuator, to identify the presence of protein, DNA, RNA, or miRNA. The fluorescein isothiocyanate (FITC) probe and biotinylated probe are designed for a two-pronged approach to the detection of the urinary miR-21 and miR-141, both of which have demonstrated significance in the development and progression of colorectal cancer, a leading cause of mortality and morbidity. The remainder of the apparatus is composed of a modified screen-printed carbon electrode (SPCE), to which the probes adhere, that transduces signals via the redox reaction between H 2 O 2 and HRP, measured with chronoamperometry and cyclic voltammetry. The precise nature of our ultra-non-invasive biosensor makes for a highly sensitive and practical cancer detector, concluded by the significance when establishing disease presence (miR-21 p -value = 0.0176, miR-141 p -value = 0.0032), disease follow-up (miR-21 p -value = 0.00154, miR141 p -value < 0.0005), and even disease severity. This article hopes to emphasize the potential of an additional clinical tool for the management of colorectal cancer.
Keyphrases
- label free
- quantum dots
- living cells
- gold nanoparticles
- papillary thyroid
- sensitive detection
- cell proliferation
- long non coding rna
- fluorescent probe
- single molecule
- squamous cell
- cardiovascular events
- small molecule
- childhood cancer
- computed tomography
- high resolution
- lymph node metastasis
- coronary artery disease
- magnetic resonance
- risk assessment
- loop mediated isothermal amplification
- squamous cell carcinoma
- molecularly imprinted
- magnetic resonance imaging
- cell free
- risk factors
- climate change
- high throughput
- mass spectrometry
- monte carlo