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Development of Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick Assay for a Rapid and Sensitive Detection of Cystic Echinococcosis in Livestock in Kenya.

Nasser Arreh BadoulJohn Maina KagiraFlorence Ng'ong'aHunduma Dinka
Published in: Journal of tropical medicine (2022)
Our results indicated that the developed LAMP-LFD technique was more sensitive than the nested PCR assay, rapid, and easy to perform with a simple visual detection of products. Therefore, it could be an important point-of-care diagnostic tool for cystic echinococcosis.
Keyphrases
  • loop mediated isothermal amplification
  • sensitive detection
  • high throughput
  • quantum dots
  • single cell
  • real time pcr