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Diagnosis of mucormycosis using an intercalating dye-based quantitative PCR.

Jeanne BigotAlexandre GodmerLysa PrudentéCécile AngebaultEolia BrissotNaike BigeGuillaume VoiriotPierre-Louis LegerCamille Petit-HoangSarah AtallahElodie GouacheYaye SenghorStéphane ValotChristophe HennequinJuliette Guitard
Published in: Medical mycology (2022)
qPCR-based diagnosis is the most reliable approach for mucormycosis. We set up a pan-Mucorales qPCR able to detect in a single reaction not less than 11 different species. Both analytical and clinical performances support its use in the clinical setting.
Keyphrases
  • high resolution
  • mass spectrometry
  • liquid chromatography