A detailed methodology for the long-term in vitro culture and analysis of three-dimensional, self-structuring bone models generated from cell lines or primary osteoblastic cell populations.

Melissa FinlayLaurence A HillGeorgiana NeagBinal PatelMiruna ChiparaHannah C LamontKathryn FrostKieran PatrickJonathan W LewisThomas NicholsonJames EdwardsSimon W JonesLiam M GroverAmy J Naylor

Published in: F1000Research (2024)

bone models that are responsive to exogenous stimuli, offering a versatile platform for conducting preclinical translatable research studies.

Keyphrases

bone mineral density
cell therapy
soft tissue
bone loss
single cell
bone regeneration
high throughput
postmenopausal women
body composition
vascular smooth muscle cells
case control
angiotensin ii
genetic diversity