High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes.
Hyun Ji AnYun KimSoojeong ChangHakchun KimJihwan SongHyunsung ParkInhee ChoiPublished in: Nature communications (2021)
Histones are closely related to the state of chromatin, and epigenetic modification of their tail results in regulation in cells. Therefore, developing various analytical tools to map the changes in position and distribution of histone modifications is helpful in studying underlying mechanisms. Herein, we propose a high-spatial and colourimetric imaging method using plasmonic nanoparticles as probes to visualize heterochromatin histone markers in a single nucleus. We visualized the reorganization between repressive histone markers, H3K9me3 and H3K27me3, caused by oncogene-induced senescence based on the scattering colours and spectral shift of plasmonic nanoprobes to longer wavelengths using their distance-dependent coupling effect. The measured scattering profiles were correlated with the computation results simulating the scattering spectra according to the arrangements and distances among the plasmonic nanoprobes. The plasmonic nanoprobe-based high-spatial hyperspectral imaging provides an advanced way to study the dynamics of histone modifications for predicting the progression of diseases or senescence.
Keyphrases
- dna methylation
- fluorescence imaging
- single molecule
- high resolution
- induced apoptosis
- dna damage
- cell cycle arrest
- energy transfer
- gene expression
- genome wide
- living cells
- endothelial cells
- small molecule
- photodynamic therapy
- cell death
- transcription factor
- optical coherence tomography
- high glucose
- density functional theory
- mass spectrometry
- visible light