OMNY-A tOMography Nano crYo stage.
Mirko HollerJürgen LindnerAna DiazM Guizar-SicairosRoger WepfM OdstrcilF R ShaikV PanneelsAndreas MenzelB SarafimovS MaagX WangV ThominetH WaltherT LachatM VitinsOliver BunkPublished in: The Review of scientific instruments (2018)
For many scientific questions gaining three-dimensional insight into a specimen can provide valuable information. We here present an instrument called "tOMography Nano crYo (OMNY)," dedicated to high resolution 3D scanning x-ray microscopy at cryogenic conditions via hard X-ray ptychography. Ptychography is a lens-less imaging method requiring accurate sample positioning. In OMNY, this in achieved via dedicated laser interferometry and closed-loop position control reaching sub-10 nm positioning accuracy. Cryogenic sample conditions are maintained via conductive cooling. 90 K can be reached when using liquid nitrogen as coolant, and 10 K is possible with liquid helium. A cryogenic sample-change mechanism permits measurements of cryogenically fixed specimens. We compare images obtained with OMNY with older measurements performed using a nitrogen gas cryo-jet of stained, epoxy-embedded retina tissue and of frozen-hydrated Chlamydomonas cells.
Keyphrases
- electron microscopy
- high resolution
- high speed
- mass spectrometry
- induced apoptosis
- ionic liquid
- cell cycle arrest
- physical activity
- deep learning
- photodynamic therapy
- optical coherence tomography
- room temperature
- convolutional neural network
- diabetic retinopathy
- magnetic resonance imaging
- health information
- single molecule
- magnetic resonance
- social media
- single cell
- cell proliferation
- reduced graphene oxide