Design of 8-mer Peptides that Block Clostridioides difficile Toxin A in Intestinal Cells.

Sudeep Sarma Carly M CatellaEllyce T San PedroXingqing Xiao Deniz Durmusoglu Stefano Menegatti Nathan C Crook Scott T Magness Carol K Hall

Published in: bioRxiv : the preprint server for biology (2023)

esign (PepBD) algorithm, molecular-level simulations, a rapid screening assay to evaluate peptide:toxin binding, a primary human cell-based assay, and surface plasmon resonance (SPR) measurements to develop peptide inhibitors that block Toxin A in small intestinal and colon epithelial cells. Importantly, our designed peptide, SA1, bound toxin A with nanomolar affinity and blocked toxicity in colon cells.

Keyphrases

escherichia coli
induced apoptosis
cell cycle arrest
high throughput
endothelial cells
oxidative stress
machine learning
single cell
endoplasmic reticulum stress
deep learning
cell death
stem cells
signaling pathway
cell proliferation
bone marrow
single molecule
binding protein
quantum dots